• 
  From the Analysis app, click the Pipelines tab. Click + New Pipeline.
• 
  Enter "260:280 Purity Test" in the Name field. Click the blank Task box.
• 
  Start entering "260" and select "260-280 Purity Test" from the auto-complete list.
• 
  Click Save.

Test Pipeline

• 
  Click Run.
• 
  Enter "DNA" for the material parameter. Enter "1.9" for the ratio parameter.
• 
  Click Run.
• 
  Click the Monitor tab. Click the "260:280 Purity Test" row for the DNA/1.9 run. The Pipeline Inspector on the right displays basic information about the executed Pipeline.
• 
  To navigate the results:
  
  Click the vertical ellipsis and then click View Details. This will redirect you to a page that looks similar to the Pipeline Builder. The key difference is that the Run Details page displays actual details from the run. Click the Task (the "260-280 Purity Test" box) to view run-time details, including associated Task Files. The Task Inspector on the right has links to the complete Task Script (as executed on the server); stderr and stdout for the script; and also a link to the Registered File, Result Report.
• 
  Click Result Report... to view the contents of the Registered File. You have now successfully tested the Pipeline.

• 
  From the Analysis app, click Pipelines. Click Edit on the 260:280 Purity Test row.
• 
  Click the Reports tab to access the Report Builder.
• 
  From the Report Elements list, drag "HTML file" to the canvas area and drop it on the Drag and drop a new report element here field.
• 
  From the Registered Files list, drag "Result Report (Step 1: 260-280)" to the canvas area and drop it on the Drag and drop a registered file here field.
• 
  Click Save.

Test Updated Pipeline

• 
  From the Analysis app, click the Pipelines tab. Click Run on the 260:280 Purity Test row.
• 
  Enter "RNA" for the material parameter. Enter "1.5" for the ratio parameter. Click Run.
• 
  Click the Monitor tab. Click the new "260:280 Purity Test" row for the RNA/1.5 run. Click View from the Pipeline Inspector on the right to view the RNA Report. The Report shows a failed RNA purity test.

• 
  Open the L7|Master app. Click Protocols from the Processes menu to access the Protocols page.
• 
  Click + New Protocol.
• 
  Enter "260:280 Report" in the Display Name field. Select No under Use in manufacturing. Select Pipeline Protocol as the Protocol Type. Select 260:280 Purity Test as the Pipeline.
• 
  Observe that two new fields appear in the Field View: material and ratio. These two new fields are for data entry, which will later be performed by filling out a Worksheet in the LIMS app.
• 
  Click anywhere in the material field. The material field will be highlighted in blue. In the Field Details panel, select Picklist as the Type. Expand the Parameters section and enter "DNA" in the Enter option 0 field. Enter "RNA" in the Enter option 1 field.
• 
  Click anywhere in the ratio field. Now the ratio field will be highlighted in blue. In the Field Details panel, select Numbers only allowed as the Type.
• 
  Click Save.

• 
  Open the L7|Master app. Click Workflows from the Processes menu.
• 
  Click + New Workflow.
• 
  Enter "260:280 Purity Assay" in the Display Name field. Select No under Use in manufacturing. Click and drag the 260:280 Quantification (from the Protocol Library panel) and drop in between the default Submitted and Completed boxes.
• 
  Click and drag the 260:280 Report (from the Protocol Library panel) and drop in between the 260:280 Quantification Protocol and the default Completed box.
• 
  Click Save.

• 
  Open the Projects app. Click + New Project.
• 
  Enter "Quick Start" as the Name.
• 
  Click Save.

• 
  On the row of the Quick Start Project you just created, click + Experiment.
• 
  Verify that Workflow is selected. Select 260:280 Purity Assay from the Workflow drop-down. Enter "Hello Quick Start" as the Name. Click Next.
• 
  Click Add New Entities. Select Generic sample from the Entity Type drop-down. Enter "4" in the Number of Entities field. Verify that ESP SEQUENCE is selected from the Entity ID Sequence drop-down. Click Next.
  
  Note: Entity ID Sequence is used to generate Entity names automatically as Entities are added.
  
  
• 
  Click Save and Submit.
• 
  Click Submit Entities in the Submit Confirmation dialog.

• 
  Open the LIMS app. Click the icon to the right of Hello Quick Start (4) in the backlog. This will open the Add New Worksheet dialog.
• 
  Update the Name to "Quick Start Sheet 1".
• 
  Click Add Worksheet.
• 
  To view the Worksheet, click the Quick Start Sheet 1 link.

• 
  In the Type column, select "DNA," "DNA," "RNA," and "RNA" from the drop-down list.
• 
  In the 260:280 Ratio column, enter the values "1.7," "1.8," "1.9," and "2.0."
• 
  Select the "Complete" checkbox for each Entity.
• 
  Click Save and Continue.
• 
  In the Material column, select "DNA," "DNA," "RNA," and "RNA" from the drop-down list.
• 
  In the Ratio column, enter "1.7," "1.8," "1.9," and "2.0."
• 
  Click Save.
• 
  For the first Entity, click Start Pipeline to run the Pipeline that generates the Report. Click Run in the Run Pipeline dialog.
• 
  Entities can run simultaneously so click Start Pipeline and then Run in the Run Pipeline dialog for each of the remaining Entities. All Complete checkboxes will be checked and the Start column will say Done once the Pipelines are finished running.
  
  Note: To monitor Entities as they move through an Experiment, open the Projects app and click on the link for Hello Quick Start in the Experiments tab. Observe that the 4 Entities have advanced to the Completed stage.
  
  Click on the Protocol box for the 260:280 Report. Observe that the Reports are directly accessible from the Inspector. 

• 
  Open the L7|Master app. Click Workflows from the Processes menu.
• 
  Click the 260:280 Purity Assay Workflow.
• 
  Click the 260:280 Report Protocol box. Click + Create for the material field to create a Data Link to connect the values in this Protocol to the values entered in the 260:280 Quantification Protocol.
• 
  In the Create Data Link dialog, select 260:280 Quantification as the Protocol. Select Type as the Column (the selected column is where the data will be linked). Click Add Below to generate an L7|ESP Expression based on the Protocol and Column selected. Click Save.
  
  Hint
  Note that the L7|ESP Expression Language previously used to create parameters when setting up the Pipeline can also be used in other parts of L7|ESP. In this case, you can set the value of the material field to be an Expression that references a value in another Protocol.
  
  
• 
  Click + Create for the ratio field.
• 
  In the Create Data Link dialog, select 260:280 Quantification as the Protocol. Select 260:280 Ratio as the Column. Click Add Below. Click Save.
• 
  Observe that both fields are now linked. Click Save to save the updated Workflow.